LONG TERM CLASSICAL METHODS FOR THE PRESERVATION OF XANTHAN GUM-PRODUCING - Xanthomonas arboricola pathovar pruni STRAINS

The maintenance of viable and stable Xanthomonas cells is crucial for the xanthan reliable research industrial production. method, storage recovery conditions should preserve both viability phenotypical genotypical features. Here, effectiveness classical methods on long-term preservation different arboricola pathovar pruni strains was to determine. Strains were preserved by monthly sub-culturing in solid medium lyophilization. After 12 years strains, assessed, as well their productive capacity viscosity gum produced these kept lyophilization sub-culturing. Among lyophilized only those stored at -18 °C after years. poorly affected lyophilization, passage cultures into a nutrition being sufficient them return normal metabolism. synthesized method-dependent higher strains. work its findings are new original because this topic has never been published before. results obtained allow breaking paradigms regarding Xanthomonas.